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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

1.2K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
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Immunoprecipitation01:20

Immunoprecipitation

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Related Experiment Video

Updated: Sep 16, 2025

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

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Purification of Monoclonal Antibodies Using Chromatographic Methods: Increasing Purity and Recovery.

Elnazalsadat Jafarzadeh Chehraghi1, Parvin Akbarzadehlaleh1, Karim Shamsasenjan2

  • 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

Advanced Pharmaceutical Bulletin
|July 10, 2025
PubMed
Summary

Monoclonal antibody (mAb) purification is vital for safe therapies. This review covers current and emerging chromatographic techniques, like multi-modal ligands and continuous processing, to improve mAb production efficiency and reduce costs.

Keywords:
Bio-SeparationChromatographyMonoclonal antibodyPEGylated monoclonal antibodyPurification

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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Protein Purification
  • Therapeutic Antibody Development

Background:

  • Monoclonal antibodies (mAbs) are critical in biopharmaceutical research and therapy due to their targeted action.
  • Effective purification of mAbs is essential to remove impurities, ensuring product safety and efficacy.
  • Chromatographic methods are the cornerstone of mAb purification, leveraging unique mAb properties.

Purpose of the Study:

  • To review current chromatographic techniques for monoclonal antibody (mAb) purification.
  • To explore recent advancements and emerging trends in mAb purification strategies.
  • To highlight the importance of method selection based on mAb characteristics and desired product quality.

Main Methods:

  • Overview of established chromatographic techniques: affinity, ion exchange, and hydrophobic interaction chromatography (HIC).
  • Discussion of innovative approaches including multi-modal ligands and membrane adsorbers.
  • Exploration of continuous processing in mAb purification.

Main Results:

  • Current methods effectively purify mAbs by exploiting properties like antigen-binding, charge, and hydrophobicity.
  • Emerging trends like multi-modal ligands and continuous processing promise enhanced efficiency and cost reduction.
  • Optimized purification strategies are key to developing safe and effective mAb-based therapies.

Conclusions:

  • Chromatographic techniques are indispensable for high-quality mAb production.
  • Advancements in purification technologies are crucial for improving efficiency, selectivity, and cost-effectiveness.
  • Tailoring purification methods to specific mAb properties and quality requirements is paramount.