Bacterial Foreignization Nanosystem Elicits Multi-Phenotypic T Cells for Antitumor Immunity

Area of Science:

• Immunology
• Biotechnology
• Nanomedicine

Background:

• Tumor cells evade T-cell attacks by altering immunogenicity (adjuvanticity, antigenicity, reactogenicity).
• Effective cancer immunotherapy requires overcoming tumor-induced immune suppression.

Purpose of the Study:

• To develop a bacterial outer membrane vesicle (OMVs)-based nanosystem to reshape tumor immunogenicity and elicit robust T-cell responses.
• To enhance tumor antigenicity, adjuvanticity, and reactogenicity for improved cancer immunotherapy.

Main Methods:

• Engineered OMVs with vesicular stomatitis virus G-protein for tumor cell fusion.
• Delivered siPD-L1 directly into tumor cell cytoplasm via OMV fusion.
• Evaluated therapeutic efficacy in murine cancer models.

Main Results:

• OMV-mediated fusion

Conclusions:

• Engineered OMVs effectively reprogram tumor immunogenicity, enhancing adaptive immunity and immune memory.
• This strategy demonstrates significant therapeutic potential for suppressing bilateral and metastatic tumors.