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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
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Epi4Ab: a data-driven prediction model of conformational epitopes for specific antibody VH/VL families and CDRs

Nhan Dinh Tran1, Krithika Subramani1, Chinh Tran-To Su1

  • 1Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), Matrix, Singapore.

Mabs
|July 10, 2025
PubMed

Antibodies recognize antigens via complementary and structurally dependent mechanisms. Therefore, inclusion of antibody inputs is crucial for accurate epitope prediction. Given the limited availability of antibody-antigen complex structures, any epitope prediction model will require minimal yet sufficient antibody inputs to ensure precise epitope identification. To address this need, we introduce Epi4Ab, an antibody-specific epitope prediction model that focuses on identifying unique in-contact antigen residues for a given antibody. Epi4Ab requires minimal antibody inputs, specifically VH/VL families and complementarity-determining region sequences.

Keywords:
Antibody-specific epitope predictionCDR sequencesVH-VL familiesconformational epitopegraph-based modelmachine learning

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