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Related Experiment Video

Updated: Sep 16, 2025

Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience
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Implementation of an Undergraduate Laboratory-Based Mammalian Genome Editing Course.

Isabelle Guerra1, Karin Jensen2, Pablo Perez-Pinera1,3,4,5,6

  • 1Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.

The CRISPR Journal
|July 10, 2025
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Summary

This study developed a practical laboratory course for genome editing, covering gene knockout, activation, repression, and base editing in mammalian cells. The course effectively prepares biology students for careers in genome engineering and related fields.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Genome engineering technologies offer precise genetic manipulation in living cells.
  • These technologies are increasingly vital across biology and biomedical fields.
  • Existing curricula lack sufficient hands-on laboratory courses for genome editing.

Purpose of the Study:

  • To develop and implement a comprehensive semester-long laboratory course on genome editing.
  • To equip students with practical skills in gene knockout, activation, repression, and base editing.
  • To prepare students for experimental design, troubleshooting, scientific writing, and presentations.

Main Methods:

  • Designed a semester-long laboratory course curriculum.
  • Included modules on gene knockout, gene activation, gene repression, and base editing in mammalian cells.
  • Integrated training in experimental design, troubleshooting, manuscript writing, and scientific presentations.

Main Results:

  • Students gained practical skills in various genome editing techniques.
  • The course successfully prepared students to design and troubleshoot experiments.
  • Course evaluations confirmed students' readiness for careers in genome engineering and related fields.

Conclusions:

  • A dedicated laboratory course is essential for teaching practical genome editing skills.
  • This course effectively bridges the gap between theoretical knowledge and practical application.
  • Graduates are well-prepared for advanced studies and careers in biotechnology and bioengineering.