Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

18.6K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
18.6K
Labeling DNA Probes03:31

Labeling DNA Probes

8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA writes the nucleolus.

Cell research·2026
Same author

A first-in-class small-molecule inhibitor targeting AVIL exhibits safety and antitumor efficacy in preclinical models of glioblastoma.

Science translational medicine·2026
Same author

GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling.

Nature·2026
Same author

Simultaneous Label-free Imaging of Nucleolar Dynamics and Subcellular Metabolic Shifts Across Tissue Contexts.

bioRxiv : the preprint server for biology·2025
Same author

At The Interface: Small-Molecule Inhibitors of Soluble Cytokines.

Chemical reviews·2025
Same author

RNA-binding proteins and glycoRNAs form domains on the cell surface for cell-penetrating peptide entry.

Cell·2025

Related Experiment Video

Updated: Sep 16, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.1K

Chemical Probe Discovery for DEAD-Box RNA-Binding Protein DDX21 Using Small-Molecule Microarrays.

Toshihiko Aiba1,2, Eliezer Calo1,3, Angela N Koehler1,2,4,5

  • 1Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

ACS Chemical Biology
|July 10, 2025
PubMed
Summary

Researchers identified KI-DX-014, a small molecule that inhibits DDX21 RNA binding. This compound modulates DDX21's functions, offering a new approach for targeting DEAD-box proteins in diseases like cancer.

More Related Videos

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.3K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.1K

Related Experiment Videos

Last Updated: Sep 16, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.1K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.3K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.1K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • RNA Metabolism

Background:

  • DEAD-box ATPases are crucial for RNA metabolism and biomolecular condensate regulation.
  • Dysregulation of DEAD-box proteins is linked to cancer and neurodegenerative diseases.
  • These proteins have been considered
  • undruggable
  • hindering therapeutic development.

Purpose of the Study:

  • To develop a method for identifying small molecules that target DEAD-box proteins.
  • To investigate DDX21 as a therapeutic target.
  • To characterize the effects of a newly discovered inhibitor on DDX21 function.

Main Methods:

  • Developed a lysate-based small-molecule microarray platform.
  • Screened for compounds that bind to DDX21.
  • Assessed the impact of the identified compound (KI-DX-014) on DDX21's ATPase activity, condensate formation, and interaction with P-TEFb.

Main Results:

  • Discovered KI-DX-014, a small molecule that inhibits DDX21's interaction with RNA.
  • KI-DX-014 modulated DDX21's RNA-dependent functions, including ATPase activity and biomolecular condensate formation.
  • KI-DX-014 inhibited P-TEFb release from the 7SK snRNP complex, suppressed RNA polymerase II CTD phosphorylation, and caused developmental defects in zebrafish.

Conclusions:

  • Established a novel platform for targeting RNA-binding proteins like DDX21.
  • KI-DX-014 serves as a chemical probe to study DDX21's biological roles.
  • These findings open new therapeutic avenues for diseases involving DDX21 dysregulation.