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Updated: Sep 16, 2025
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
Published on: October 13, 2015
Uncovering Transient Protein Interactions in the Yersinia enterocolitica Type III Secretion System Using
Katherine Lapis Pintor1, Andreas Diepold1,2, Georgia Angelidou1
1Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Abstract:
Many critical protein-protein interactions are transient, making them challenging to study using established methods. This limitation is particularly evident in transport processes like bacterial secretion systems, where the interactions between the export machinery and the cargo proteins are inherently dynamic. In this protocol, we describe a sensitive method that allows for the identification of such interactions using the example of SycH, the chaperone of the bacterial type III secretion system (T3SS) effector YopH. Using this method in live Yersinia enterocolitica, we identified known as well as previously uncharacterized stable and transient interactors of this protein.
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