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Updated: Sep 16, 2025

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
Characterization of the Protein Interactome of Membrane-Bound Transcription Factors Using TurboID-Based Proximity
Jonas De Backer1,2, Lindsy De Veirman1,2, Inge De Clercq3,4
1Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium, Technologiepark 71.
Abstract:
A wide range of technologies have been developed to study protein-protein interactions and to map comprehensive protein interaction networks. In recent years, advances in proximity labeling methods, coupled to protein identification by mass spectrometry, have led to exponential growth in the popularity of these methods. In this chapter, we present a detailed protocol using TurboID in Arabidopsis thaliana seedlings, using membrane-bound transcription factors as a case study. Additionally, we outline the workflow for analyzing mass spectrometry data to identify putative protein interactors.
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