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Published on: August 31, 2016
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BioID2-Based Proximity Labeling in Adult Zebrafish
1Department of Biological Sciences, University of Denver, Denver, CO, USA. mira.pronobis@du.edu.
Methods in Molecular Biology (Clifton, N.J.)
|July 10, 2025
Summary
This study establishes in vivo proximity labeling in adult zebrafish, enabling precise mapping of protein interactions within a living vertebrate system. This technique advances the study of complex biological networks in a model organism with significant regenerative capabilities.
Area of Science:
- Biochemistry
- Molecular Biology
- Systems Biology
Background:
- Proximity labeling is crucial for mapping protein networks in native biological contexts.
- Current applications are largely confined to cell culture, limiting in vivo studies.
- Zebrafish offer a versatile vertebrate model for in vivo research due to regeneration and genetic tractability.
Purpose of the Study:
- To establish and validate an in vivo proximity labeling protocol in adult zebrafish.
- To enable precise identification of protein interactomes within a living vertebrate system.
- To provide a foundation for studying protein networks in vivo using zebrafish.
Main Methods:
- Development of a protocol for in vivo proximity labeling in adult zebrafish.
- Utilizing zebrafish's genetic tractability for targeted labeling.
- Application of proximity labeling techniques in a whole-organism context.
Main Results:
- Successful establishment of in vivo proximity labeling in adult zebrafish.
- Demonstration of precise protein interactome mapping within the zebrafish model.
- Validation of the protocol for studying protein networks in vivo.
Conclusions:
- In vivo proximity labeling is feasible and effective in adult zebrafish.
- This protocol expands the application of proximity labeling to vertebrate models.
- Zebrafish serve as a powerful model for in vivo interactome studies.
