Investigation of molecular patterns present in RIPK1 and systemic approach to determine its differential expression...

Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) regulates the pro-survival NF-κB signaling and death via necroptosis in response to various inflammatory and pro-death stimuli, leading to various human diseases. It is linked with the pathology of neuroinflammation and implicated in neurodegenerative disorders. Considering the imperative role of RIPK1 kinase in neuronal health, molecular patterns, effects of evolutionary forces on composition and codon bias, and tissue-wise expression pattern analysis in 12 different parts of the brain was done using a systematic approach in the RIPK1 kinase transcripts. Nucleotide G experienced the highest mutational force and variance despite the presence of nucleotide A as the most abundant nucleotide in the composition. In different parts of the brain, the average CAI values of different transcripts were different, and the highest expression was present in the Cerebellar Hemisphere, while the lowest was in the Cortex. The expression level present in the brain Cortex was significantly different (p < 0.001) from all other envisaged 11 brain tissues. Various physical properties of protein were envisaged. The present work revealed that a few of the protein properties exhibited correlation with each other. Dinucleotides ApA, ApG, CpA, and GpA were overrepresented, while CpG, GpT, TpA, TpC, and TpT were underrepresented, suggestive of the influence of composition on dinucleotide bias. Studying molecular patterns might help in the future in understanding the etiology of brain disease caused by RIPK1.