Robust and Versatile OPH-Cu Phosphate Hybrid Nanoflower for Organophosphorus Compound Degradation

Organophosphorus hydrolase (OPH), a promising biocatalyst for the degradation of organophosphorus compounds, faces application limitations due to its structural fragility. To address this, we developed a novel bioinorganic hybrid nanoflowers (HNFs) system by integrating OPH with copper phosphate via a self-assembly strategy. The HNFs exhibited significant catalytic robustness across a broad pH and temperature range. This hybrid system demonstrated enhanced tolerance to organic solvents and proteases, retaining 81% of its initial activity after five catalytic cycles. Furthermore, we successfully tandem-catalyzed the enzymatic hydrolysis and metal-catalyzed hydrogenation reactions using OPH and copper phosphate as the bio- and metal catalysts, respectively, thereby completely converting the toxic substrate into low-toxicity products. This bifunctional catalytic system offers a novel solution for the deep detoxification of organophosphorus pollutants.