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Ptychoscopy: a user friendly experimental design tool for ptychography
Radim Skoupy1, Elisabeth Müller1, Timothy J Pennycook2
1PSI Center for Life Sciences, Paul Scherrer Institute, PSI, Villigen, 5232, Switzerland.
Scientific Reports
|July 10, 2025
Summary
This study introduces ptychoScopy, a Python tool simplifying electron ptychography experimental design. It helps researchers optimize parameters for high-quality imaging in both physical and life sciences.
Area of Science:
- * Physical Sciences
- * Life Sciences
- * Materials Science
Background:
- * Electron ptychography is an advanced diffractive imaging technique.
- * It offers high resolution, superior contrast, and efficient dose usage.
- * Successful reconstruction depends on experimental parameters and algorithms.
Purpose of the Study:
- * To introduce ptychoScopy, a Python-based tool for experimental design in electron ptychography.
- * To simplify parameter selection for successful ptychographic reconstructions.
- * To guide researchers in diverse scientific fields.
Main Methods:
- * Development of ptychoScopy, a Python software tool.
- * Analysis of electron ptychography using a [Formula: see text] sample.
- * Investigation of key parameters: probe convergence angle, defocus, electron dose, and sampling.
- * Comparison of direct and iterative reconstruction algorithms.
Main Results:
- * Demonstrated the influence of probe convergence angle, defocus, and electron dose on reconstruction quality.
- * Evaluated the impact of real and reciprocal space sampling on reconstruction speed and quality.
- * Showcased ptychoScopy's utility in simplifying experimental setup.
Conclusions:
- * PtychoScopy effectively aids in selecting optimal parameters for electron ptychography.
- * The tool facilitates successful experimental design across various scientific disciplines.
- * It empowers researchers to achieve high-quality ptychographic reconstructions more efficiently.

