Bridging molecular and cellular neuroscience with proximity labeling technologies

Area of Science:

• Neuroscience
• Molecular Biology
• Cell Biology

Background:

• Proximity labeling (PL) techniques are crucial for understanding molecular interactions in neural circuits.
• Foundational tools like BioID and APEX, along with innovations like TurboID and light-activated systems, enable precise mapping of protein-protein interactions in living cells.

Purpose of the Study:

• To review the advancements and applications of proximity labeling techniques in neuroscience.
• To highlight the role of PL in deciphering molecular mechanisms underlying neural connectivity in health and disease.

Main Methods:

• Review of existing literature on proximity labeling techniques and their applications.
• Integration of PL with multi-omics and advanced imaging strategies.

Main Results:

• PL has identified dynamic protein networks involved in synaptic remodeling, calcium signaling, and neurological disorders.
• PL aids in comprehending brain's molecular architecture and uncovering therapeutic targets.
• Integration with multi-omics and imaging enhances the decoding of neural network interplay.

Conclusions:

• Proximity labeling technologies are pivotal for unraveling the complexity of brain networks.
• Continued evolution of PL promises deeper insights into the molecular basis of neural connectivity.
• PL bridges molecular and cellular neuroscience, offering a powerful framework for research.