Exploring the impact of diet, sleep, and metabolomic pathways on Glaucoma subtypes: insights from Mendelian...

Glaucoma is a leading cause of irreversible blindness, influenced by systemic and lifestyle factors. This study investigates the causal relationships between dietary habits, sleep traits, amino acids, metabolites, and inflammatory factors with glaucoma subtypes using Mendelian randomization (MR) and validates findings through cross-sectional analysis.

MR analysis assessed the causal effects of 226 dietary factors, 11 sleep traits, 20 amino acids, 1400 metabolites, and 91 inflammatory factors on five glaucoma subtypes (NTG, POAG, PACG, NVG, XFG). Mediation MR analysis explored the role of amino acids and inflammatory factors in these relationships. Validation was conducted using NHANES cross-sectional data.

High-fat, high-calorie diets increased glaucoma risk, while antioxidant-rich foods and better sleep quality reduced it. Key mediators included proline, tyrosine, IL-1 A, and PDL1. NHANES data confirmed lower intake of vitamins A and C, higher water consumption among glaucoma patients, and significant sleep-related associations.

Our findings highlight the role of balanced diets and optimized sleep patterns in glaucoma prevention and management. This study provides evidence for targeted lifestyle interventions focusing on metabolic and inflammatory pathways to mitigate glaucoma risk.