Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Preparation and performance evaluation of a novel Tween 80-based microemulsion system for the remediation of petroleum hydrocarbon-contaminated soil using soil washing test.

Environmental research·2026

Same author

Decoding the role of chromatin context in the off-target effects of CRISPR gene editing with EGOLD.

Cell discovery·2026

Same author

Microemulsion flushing of petroleum hydrocarbons in porous media: Microfluidic insights into residual oil morphologies and removal mechanisms.

Journal of environmental management·2026

Same author

Electrostatic remodeling of TadA8e* eliminates ABE8e genome-wide off-target effects.

Trends in biotechnology·2026

Same author

Large-scale parallel characterization of RNA-guided nuclease activity and specificity.

Science bulletin·2026

Same author

Chemical reprogramming directly resets mouse post-implantation epiblast cells to a totipotent state.

Cell research·2026

Search research articles

Related Experiment Video

Updated: Sep 16, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

SuperDecode: A versatile toolkit for mutation analysis in genome editing.

Guoying Hua¹, Chenfei He¹, Erwei Zuo¹

¹Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120 China.

|July 11, 2025

Summary

This summary is machine-generated.

SuperDecode enhances CRISPR-Cas editing efficiency quantification by offering local processing and batch capabilities for large sequencing files. This novel computational framework improves data analysis throughput and operational flexibility in life sciences research.

Keywords:

Amplicon CRISPR/Cas Genome editing Mutation analysis SuperDecode

More Related Videos

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Published on: September 28, 2022

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Published on: September 10, 2021

Related Experiment Videos

Last Updated: Sep 16, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Published on: September 28, 2022

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Published on: September 10, 2021

Area of Science:

CRISPR-Cas gene editing technologies
Computational biology and bioinformatics
Genomics and functional genomics

Background:

CRISPR-Cas systems are revolutionizing life sciences, enabling advances in genomics and therapeutics.
Current computational tools for quantifying CRISPR editing efficiency face limitations in data processing, throughput, and flexibility.
Addressing these limitations is crucial for advancing CRISPR-based research and applications.

Discussion:

SuperDecode is a novel computational framework designed to overcome existing limitations in CRISPR editing efficiency quantification.
It addresses challenges related to data processing, analytical throughput, and operational flexibility.
The framework facilitates more robust and efficient analysis of CRISPR experimental outcomes.

Key Insights:

SuperDecode enables local processing of large sequencing files, enhancing data security and speed.
The framework supports batch processing, significantly increasing analytical throughput for high-volume experiments.
Diversified operational functions provide flexibility for various research needs and experimental designs.

Outlook:

SuperDecode is expected to accelerate CRISPR-Cas research by providing a more efficient and flexible quantification tool.
Its capabilities can facilitate the development and optimization of CRISPR-based therapeutics.
The framework has the potential to become a standard tool in genomics and functional genomics laboratories.