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Related Experiment Videos

Fatty acid binding proteins from heart.

J F Glatz, R J Paulussen, J H Veerkamp

    Chemistry and Physics of Lipids
    |August 30, 1985
    PubMed
    Summary
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    Heart fatty acid-binding protein (FABP) concentration is comparable to liver FABP. Heart FABP plays a role in myocardial fatty acid metabolism, with levels varying by sex and diurnal cycle.

    Area of Science:

    • Biochemistry
    • Cardiology
    • Molecular Biology

    Background:

    • Heart fatty acid-binding protein (FABP) concentration is comparable to liver FABP when measured by binding assay.
    • Discrepancies in FABP concentration arise from differing chemical forms and properties between liver and heart FABP.
    • The ratio of fatty acid bound per purified protein molecule is low (one or less).

    Purpose of the Study:

    • To investigate the characteristics and functional significance of heart FABP.
    • To explore the relationship between heart FABP and myocardial fatty acid metabolism.
    • To determine factors influencing heart FABP levels.

    Main Methods:

    • Fatty acid-binding assays to determine FABP concentration.
    • Use of anti-liver FABP antibodies to highlight differences.

    Related Experiment Videos

  • Isolation and characterization of human heart FABP.
  • Studies on rat heart mitochondria and FABP oxidation.

    • Main Results:

    • Heart FABP concentration is comparable to liver FABP via binding assays.
    • Rat heart FABP levels are influenced by sex and diurnal cycles, but not starvation or clofibrate.
    • Human heart FABP was isolated with an 11% yield and exhibits broad fatty acid binding.
    • Rat heart mitochondria can oxidize FABP-bound fatty acids.

    Conclusions:

    • Heart FABP is distinct from liver FABP, with unique properties and roles.
    • Heart FABP is integral to myocardial fatty acid metabolism in both normal and pathological states.
    • Factors like sex and diurnal rhythm significantly impact heart FABP levels.