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Related Concept Videos

Introduction to the Human Microbiota01:22

Introduction to the Human Microbiota

209
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
209

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This study shows that periexpander fluid from tissue expanders can be used to analyze the breast microbiome after mastectomy. Understanding this microbiome is key to reducing implant infection risks.

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Area of Science:

  • Microbiology
  • Plastic Surgery
  • Oncology

Background:

  • The breast microbiome's role in reconstruction is understudied.
  • Periexpander fluid offers a method to sample the local breast environment.
  • Previous research utilized 16S rRNA sequencing for breast microbiome analysis.

Purpose of the Study:

  • To assess the feasibility of obtaining microbiome data from periexpander fluid.
  • To explore the relevance of periexpander fluid microbiome in clinical implant infections.
  • To define the local breast microbiome in patients undergoing reconstruction.

Main Methods:

  • Pilot study involving patients undergoing mastectomy and 2-stage implant reconstruction.
  • Collection of periexpander fluid from dual-port tissue expanders.
  • 16S rRNA sequencing to analyze the microbiome of collected fluid samples.

Main Results:

  • Microbiome data was successfully obtained from all periexpander fluid aspirates.
  • A diverse range of bacterial genera were identified.
  • Common genera included *Pseudomonas*, *Corynebacterium*, *Phenylobacterium*, *Acinetobacter*, and *Staphylococcus*.

Conclusions:

  • Microbiome sequencing of periexpander aspirates is feasible for defining the breast tissue environment.
  • Optimizing the breast microbiome balance is crucial for minimizing infection risk.
  • Further research is needed to understand the clinical implications of the breast microbiome in reconstruction.