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Thiocoumarin Ethenylene-Bridge Dicyanoisophorone Skeleton: A Strategy for Ratiometric Fluorescent Detection of
Mengyao Yuan1, Yongyu Du1, Kuoxi Xu1
1Henan Engineering Research Center of Industrial Recirculating Water Treatment, College of Chemistry and Molecular Sciences, Henan University, Kaifeng 475004, China.
Abstract:
Herein, an ethylene-bridge bond of diethylaminocoumarin and dicyanoisophorone unit fluorescence probe was developed for the specific detection of HClO. The sensing mechanism involves HClO-induced cleavage of the N,N-dimethylthiocarbamate group, oxidation-triggered ethylene bridge rearrangement, ultimately leading to the formation of a unique thiophene ring structure. The probe was successfully applied for simultaneous detection of HClO in beverage and food samples. Moreover, the probe facilitated high-contrast imaging of both endogenous and exogenous HClO in live cells and in vivo mouse models, offering a powerful tool for elucidating HClO-associated physiological and pathological mechanisms.
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