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Related Experiment Video

Updated: Sep 16, 2025

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
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Human Extravillous Trophoblasts Require SRC-2 for Sustained Viability, Migration, and Invasion.

Vineet K Maurya1, Pooja Popli2, Bryan C Nikolai1

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Cells
|July 11, 2025
PubMed
Summary

Steroid receptor coactivator-2 (SRC-2) is vital for human extravillous trophoblast cells, supporting their viability, motility, and invasion. SRC-2 regulates genes, including WNT9A, crucial for placental development and function.

Keywords:
HTR-8/SVneoWNT family member 9A (WNT 9A)human extravillous trophoblaststeroid receptor coactivator-2 (SRC-2)transcriptomics

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Area of Science:

  • Reproductive Biology
  • Molecular Endocrinology
  • Cellular Biology

Background:

  • Defective placentation contributes to gestational complications like preeclampsia and intrauterine growth restriction.
  • Extravillous trophoblast (EVT) cell viability, migration, and invasion are critical for placental development, but molecular regulators are not fully understood.

Purpose of the Study:

  • To investigate the role of steroid receptor coactivator-2 (SRC-2) in human extravillous trophoblast (EVT) cell function.
  • To identify the SRC-2-dependent molecular pathways regulating EVT cellular processes.

Main Methods:

  • Utilized the immortalized human EVT cell line HTR-8/SVneo.
  • Performed genome-scale transcriptomics to identify the SRC-2-dependent transcriptome.
  • Investigated the regulatory role of SRC-2 on WNT family member 9A (WNT9A) expression and function.

Main Results:

  • Steroid receptor coactivator-2 (SRC-2) is essential for EVT cell viability, motility, and invasion.
  • An SRC-2-dependent transcriptome was identified, encoding proteins vital for placental development and function.
  • WNT9A was found to be regulated by SRC-2 and crucial for maintaining SRC-2-dependent EVT functions.

Conclusions:

  • SRC-2 plays a pivotal role in regulating essential cellular functions of human extravillous trophoblasts.
  • The SRC-2-dependent transcriptome provides insights into molecular determinants of placental development.
  • WNT9A is a key downstream effector of SRC-2 in maintaining EVT cellular functions.