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Integrated Microbiome-Metabolome Analysis Reveals Intestine-Liver Metabolic Associations in the Moustache Toad
Shui-Sheng Yu1,2, Jing-Wen Xiang1, Lin Zhang3
1Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Lishui 323000, China.
Animals : an Open Access Journal From MDPI
|July 12, 2025
Summary
The amphibian gut microbiome influences host metabolism. This study reveals distinct intestinal microbial communities and their links to liver metabolites in Leptobrachium liui, suggesting a functional gut-liver axis.
Area of Science:
- Microbiology
- Metabolomics
- Amphibian Biology
Background:
- The intestinal microbiota plays a crucial role in host metabolic homeostasis through bioactive metabolite production.
- Microbial communities aid digestion, immune function, and physiological balance, enhancing adaptation.
Purpose of the Study:
- To investigate intestinal microbiota-liver metabolic interactions in the amphibian Leptobrachium liui.
- To identify correlations between gut microbial composition and hepatic metabolites.
Main Methods:
- 16S rRNA gene sequencing for microbial community analysis.
- Non-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) for metabolomics.
- Analysis of gut segment-specific (small intestine vs. large intestine) microbial and metabolic profiles.
Main Results:
- Comparable alpha diversity but distinct microbial structures in the small intestine (SI) and large intestine (LI).
- SI microbiota dominated by Enterobacteriaceae; LI by Chitinophagaceae.
- Segment-specific microbe-metabolite correlations observed, with significant links between bacterial families and hepatic metabolites involved in fatty acid, amino acid, and energy metabolism.
Conclusions:
- The study highlights segment-specific gut microbial communities in Leptobrachium liui.
- Identified potential gut-liver axis associations through microbe-metabolite correlations.
- Provides foundational insights into amphibian host-microbe-metabolite interactions.
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