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Updated: Sep 16, 2025

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miR-195-5p Suppresses KRT80 Expression Inducing Cell Cycle Arrest in Colon Cancer

Emanuele Piccinno1, Viviana Scalavino1, Nicoletta Labarile1

  • 1National Institute of Gastroenterology S. De Bellis, IRCCS Research Hospital, Via Turi 27, 70013 Castellana Grotte, Italy.

Cancers
|July 12, 2025
PubMed
Abstract

Insights

MicroRNA-195-5p suppresses colorectal cancer (CRC) growth by downregulating Keratin 80 (KRT80) expression. This interaction halts cancer cell cycle progression, offering a potential therapeutic target for CRC.

Area of Science:

  • Molecular biology
  • Oncology
  • Gene regulation

Background:

  • Keratins are vital for epithelial cytoskeleton integrity and cellular functions.
  • Keratin 80 (KRT80) is implicated as an oncogenic driver in various cancers.
  • The role of KRT80 in colorectal cancer (CRC) pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the regulatory relationship between miR-195-5p and KRT80 in colorectal cancer.
  • To elucidate the functional impact of this interaction on CRC cell proliferation and tumor growth.

Main Methods:

  • Bioinformatic analysis identified miR-195-5p binding sites in the KRT80 3'-UTR.
  • KRT80 expression was validated in CRC tissues and cell lines via mRNA and protein analysis.
  • Functional assays (mimic transfection, siRNA silencing, in vivo studies) assessed the impact of miR-195-5p on KRT80 and CRC progression.

Main Results:

  • miR-195-5p significantly downregulated KRT80 expression in CRC cell lines and in a mouse model.
  • KRT80 downregulation induced G1-phase cell cycle arrest and reduced G2/M populations.
  • miR-195-5p demonstrated tumor-suppressive activity by inhibiting CRC growth.

Conclusions:

  • miR-195-5p acts as a negative regulator of KRT80 in colorectal cancer.
  • This miR-195-5p/KRT80 axis represents a novel molecular mechanism with therapeutic potential for CRC treatment.
Keywords:
CRCKRT80cell cyclekeratinsmiR-195-5p

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