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Updated: Sep 16, 2025
Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
Published on: July 2, 2018
3D-Printed Alginate-Based Hydrogels with Appropriate Rheological Properties and Efficient Development of Cell
Alida Mazzoli1, Stefania Greco2, Francesca Luzi1
1Department of Science and Engineering of Matter, Environment and Urban Planning (SIMAU), Università Politecnica delle Marche, UdR INSTM, Via Brecce Bianche, 60131 Ancona, Italy.
None:
In the last years, considerable innovation has been made regarding bioprinting, particularly in the development of cell-loaded hydrogels. The specific properties of the bioinks are crucial for printing an adequate cell-laden hydrogel structure. In this research, we aimed to develop a 3D-printable hydrogel using a natural biocompatible polymer. The process is based on the use of sodium alginate subjected to calcium ion cross-linking for immediate stiffness after printing. Using the Cellink INKREDIBLE+ printer (Cellink Inc., Goteborg, Sweden), 3D structures were successfully produced. The developed bioink exhibited a viscosity suitable for extrusion printing while ensuring its structural integrity at the same time. Next, 3D spheroids developed by using bioinks were morphologically characterized by using light, a fluorescent microscope, and field emission scanning electron microscopy (FESEM). In conclusion, the properties of the construct obtained using the lab-formulated biocompatible polymer hydrogel suggest its potential use as a framework for three-dimensional cell culture, with possible applications in both fields of research and regenerative medicine.
