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Related Experiment Video

Updated: Jun 9, 2026

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

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Published on: October 29, 2015

Influenza a Virus Inhibition: Evaluating Computationally Identified Cyproheptadine Through In Vitro Assessment.

Sanja Glisic¹, Kristina Stevanovic^1,2, Andrej Perdih^2,3

¹Laboratory for Bioinformatics and Computational Chemistry, Institute of Nuclear Sciences VINCA, University of Belgrade, 11000 Belgrade, Serbia.

International Journal of Molecular Sciences

|July 12, 2025

Summary

Cyproheptadine, an H1 antagonist, shows potential as an influenza antiviral. Computational methods predicted its activity, which was confirmed through in vitro experiments, supporting drug repurposing strategies.

Keywords:

antiviral cyproheptadine drug resistance influenza virtual screening

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Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

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High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

Published on: October 30, 2021

Area of Science:

Virology
Computational Chemistry
Pharmacology

Background:

Influenza remains a significant global health challenge, necessitating novel antiviral therapies.
Computational approaches are instrumental in identifying small molecule therapeutics.
Drug repurposing offers an efficient strategy for developing new treatments.

Purpose of the Study:

To investigate the potential anti-influenza activity of cyproheptadine, a histamine H1 receptor antagonist.
To explore the utility of integrating in silico screening with experimental validation for drug discovery.
To provide proof of concept for computationally predicted antiviral potential.

Main Methods:

Utilized a combined in silico and in vitro approach.
Performed virtual screening using average quasivalence number (AQVN) and electron-ion interaction potential (EIIP) descriptors.
Conducted ligand-based pharmacophore screening and experimental in vitro evaluation.

Main Results:

In silico analysis indicated similarities between cyproheptadine and known anti-influenza agents.
Experimental evaluation confirmed the anti-influenza activity of cyproheptadine.
The study demonstrated successful integration of computational predictions and experimental validation.

Conclusions:

Cyproheptadine exhibits promising anti-influenza potential.
Computational methods, when combined with experimental validation, are valuable for early-stage drug repurposing.
This study validates the in silico prediction of antiviral activity for cyproheptadine.