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Updated: Sep 16, 2025

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A Novel Regulatory Role for RPS4Y1 in Inflammatory and Fibrotic Processes.

Karosham D Reddy1,2, Senani N H Rathnayake1,2, Sobia Idrees1

  • 1School of Life Sciences, Faculty of Science, University of Technology Sydney, Ultimo, NSW 2007, Australia.

International Journal of Molecular Sciences
|July 12, 2025
PubMed
Summary
This summary is machine-generated.

The Y-chromosome gene RPS4Y1 plays a role in asthma by influencing specific protein translation. Its absence in females may explain sex differences in asthma susceptibility and severity.

Keywords:
CRISPR-Cas9RNA-seqY-chromosomeasthmafibrosisinflammationsex differences

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Area of Science:

  • Respiratory Medicine
  • Genetics
  • Molecular Biology

Background:

  • Asthma exhibits sexual dimorphism in incidence and severity, but underlying mechanisms are unclear.
  • RPS4X and RPS4Y1 are ribosomal subunit genes linked to inflammation and asthma.
  • RPS4Y1's role in disease, particularly its molecular mechanisms, is under-investigated.

Purpose of the Study:

  • To investigate the function of RPS4Y1 in asthma-related inflammation and fibrosis.
  • To explore novel molecular mechanisms regulated by RPS4Y1.
  • To understand the contribution of RPS4Y1 to sex differences in asthma.

Main Methods:

  • CRISPR-Cas9 gene editing to create RPS4Y1 and RPS4X knockout cellular models.
  • Characterization of RPS4Y1 function in inflammation and fibrosis pathways.
  • Analysis of RPS4Y1 gene expression correlation with clinical lung function data.

Main Results:

  • No viable RPS4X knockout models were generated.
  • RPS4Y1-containing ribosomes specifically translate IL6 and tenascin-C mRNA.
  • An RPS4Y1 gene signature correlates with lung function in adult male asthma patients.

Conclusions:

  • RPS4Y1 has a functional role in asthma-relevant processes like inflammation and fibrosis.
  • RPS4Y1 regulates the translation of key asthma-associated genes.
  • The absence of RPS4Y1 protein in females may contribute to the sexual dimorphism observed in asthma.