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Development of a γ-Cyclodextrin-Based Cryogel Loaded with Trimethoprim for Acne Treatment: Design, Synthesis, and In Vitro Evaluation

  • 0Department of Drug and Health Sciences, University of Catania, V.le A. Doria 6, 95125 Catania, Italy.
International Journal of Molecular Sciences +

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July 12, 2025

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July 12, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study developed a novel cryogel patch for acne treatment using a trimethoprim (TMP) and gamma-cyclodextrin (γ-CD) complex. The patch offers sustained drug release and antibacterial activity against Staphylococcus aureus, proving biocompatible for topical application.

Area Of Science

  • Materials Science
  • Pharmaceutical Sciences
  • Biotechnology

Background

  • Host-guest complexes within cryogels show potential for topical drug delivery.
  • Enhancing drug solubility and stability is crucial for effective topical formulations.
  • Acne treatment requires localized and sustained delivery of antibacterial agents.

Purpose Of The Study

  • To design and develop a cryogel-based patch for acne treatment.
  • To create a hybrid system integrating a trimethoprim (TMP) and gamma-cyclodextrin (γ-CD) inclusion complex.
  • To evaluate the sustained release, antibacterial efficacy, and biocompatibility of the developed patch.

Main Methods

  • Synthesis of the γ-CD and TMP inclusion complex using mortar-assisted kneading.
  • Cryogel formulation via radical cryopolymerization of the complex with [2-(acryloyloxy)ethyl]trimethylammonium chloride (AETMA).
  • Characterization using FT-IR, TGA, and SEM; in vitro release studies; antibacterial assays; and cytocompatibility tests.

Main Results

  • The cryogel system demonstrated sustained release of TMP, following the Korsmeyer-Peppas diffusion model.
  • Effective antibacterial activity against Staphylococcus aureus was confirmed.
  • The formulation exhibited good cytocompatibility, indicating suitability for topical application.

Conclusions

  • The developed cryogel patch effectively delivers TMP for sustained acne treatment.
  • The hybrid material leverages γ-CD for drug reservoir function and AETMA for skin adhesion.
  • This innovative material holds significant promise for localized and prolonged topical acne therapy.

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