Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

53.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

272
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
272
What is Genetic Engineering?00:49

What is Genetic Engineering?

75.6K
Overview
75.6K
The Central Dogma01:20

The Central Dogma

28.4K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
28.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid Approach to Protein-Protein Complex Affinity Prediction Based on Language Models and Molecular Dynamics.

International journal of molecular sciences·2026
Same author

scRADAR: Dissecting intratumoral drug response heterogeneity at single-cell resolution via mechanism-guided prototype routing.

PLoS computational biology·2026
Same author

Humanized Murine Glioblastoma Models for Evaluation of Coxsackievirus Oncolytic Therapy.

Cancers·2026
Same author

Potency-enhancing mutations in E3-19K and i-leader increase the cytolytic activity of the PH20/<i>SPAM1</i>-armed oncolytic adenovirus Ad5Δ24RGD.

Molecular therapy. Oncology·2026
Same author

(Re)defining the human chromatome: an integrated meta-analysis of localization, function, abundance, physical properties, and domain composition of chromatin proteins.

Nucleic acids research·2026
Same author

DSCA-HLAII: A dual-stream cross-attention model for predicting peptide-HLA class II interaction and presentation.

PLoS computational biology·2026

Related Experiment Video

Updated: Sep 16, 2025

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.7K

Epigenome Engineering Using dCas Systems for Biomedical Applications and Biotechnology: Current Achievements,

Maxim A Kovalev1, Naida Yu Mamaeva1, Nikolay V Kristovskiy1

  • 1Department of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.

International Journal of Molecular Sciences
|July 12, 2025
PubMed
Summary

CRISPR/dCas epigenome editing offers precise gene modulation without DNA changes. This review covers its biomedical and biotech applications, highlighting innovations and challenges for future therapeutic development.

Keywords:
CRISPR/Casbiomedical applicationsbiotechnologydCasepigenetic engineeringepigeneticsepigenome editingepigenome engineering

More Related Videos

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.1K
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

13.2K

Related Experiment Videos

Last Updated: Sep 16, 2025

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.7K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.1K
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

13.2K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Epigenome engineering enables gene expression control without altering DNA sequence.
  • CRISPR/dCas systems are key tools for targeted epigenome modification.
  • This technology holds significant potential for therapeutic interventions and biotechnological advancements.

Purpose of the Study:

  • To review current achievements and future prospects of dCas-mediated epigenome editing.
  • To focus on biomedical applications while considering broader biotechnological uses.
  • To examine therapeutic potential for various diseases and applications in biotechnology.

Main Methods:

  • Review of CRISPR/dCas architectures and epigenetic editor designs.
  • Analysis of delivery methods for epigenome editing tools.
  • Examination of therapeutic potential and biotechnological applications.

Main Results:

  • Diverse CRISPR/dCas architectures and innovative epigenetic editors have been developed.
  • Significant therapeutic potential exists for hereditary, neurodegenerative, and metabolic disorders.
  • Applications span biomedicine, animal, agricultural, and industrial biotechnology.

Conclusions:

  • CRISPR/dCas epigenome editing is a powerful strategy with broad applications.
  • Challenges in delivery, specificity, and clinical translation remain.
  • Future research should focus on enhancing efficacy, safety, and practical applicability.