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Improved SERS Biosensor Constructed by PolyA-Assisted Plasmonic Au Networks and Mg2+-Mediated Amplification
Huan Liang1, Yemei Liu1, Ying Zhuo1
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University) Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
ACS Applied Materials & Interfaces
|July 12, 2025
Summary
Researchers developed a novel polyA-assisted plasmonic gold (PPA) network for highly sensitive detection of circulating tumor DNA (ctDNA). This SERS biosensor achieves a low detection limit, improving cancer monitoring capabilities.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Circulating tumor DNA (ctDNA) is a critical biomarker for cancer management.
- Low ctDNA concentrations pose challenges for accurate detection and monitoring.
- Novel amplification and detection strategies are needed for sensitive ctDNA analysis.
Purpose of the Study:
- To develop a sensitive biosensor for ctDNA detection.
- To construct polyA-assisted plasmonic gold (PPA) networks.
- To leverage surface-enhanced Raman scattering (SERS) for enhanced ctDNA quantification.
Main Methods:
- Fabrication of PPA networks using polyA and gold nanoparticles (Au NPs).
- Integration of a Mg2+-mediated amplification strategy.
- Utilization of SERS technique for signal amplification and detection.
Main Results:
- Achieved a highly sensitive SERS biosensor for ctDNA detection.
- Established a detection limit of 10.8 fmol/L for ctDNA.
- Demonstrated the robust platform for precision-engineered PPA network fabrication.
Conclusions:
- The PPA network SERS biosensor offers accurate and reliable ctDNA detection.
- This advancement facilitates effective cancer monitoring and treatment strategies.
- The developed platform shows significant potential in complex biological fluid analysis.
