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Updated: Sep 16, 2025
Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
Published on: January 15, 2016
Intratumoral Bacterial-Targeted Cascade-Responsive Sonoafterglow Nanoprobes for Immune Reprogramming and
Yanping Jiang1, Jiawei Zhang1, Jingjing Ye1
1Institute of Functional Nano & Soft Materials & Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC), Soochow University, Suzhou 215123, China.
Abstract:
Bacteria within glioblastoma (GBM) promote tumor progression and immunosuppression, while current treatments face challenges such as poor blood-brain barrier (BBB) penetration and immunosuppressive macrophages. To overcome these, we developed cascade-responsive sonoafterglow nanoprobes for bacteria-targeting, immune reprogramming, and imaging. These nanoprobes, incorporating a sonoafterglow pro-substrate, sonosensitizer, and maltodextrin, selectively targeted bacteria via the ATP-binding cassette sugar transporter pathway. Delivered through ultrasound (US)-induced BBB opening, they achieved a 1.5-fold enhancement in GBM accumulation. Upon US activation, they generated singlet oxygen (1O2), eradicating bacteria, ablating tumor cells, and reprogramming macrophages to a pro-inflammatory state secreting peroxynitrite (ONOO-). This cascade-responsive system enabled precise afterglow activation via 1O2 and ONOO- interactions, achieving real-time imaging of the tumor immune microenvironment. Integrating bacterial targeting, immune modulation, and imaging, this platform presents a promising GBM therapy.
