The Human Papillomavirus (HPV) Laboratory e-Manual: A comprehensive guide for HPV testing and research

Area of Science:

• Virology
• Public Health
• Laboratory Science

Background:

• Human Papillomavirus (HPV) vaccination and screening are key to the WHO's cervical cancer elimination strategy.
• The 2009 WHO HPV Laboratory Manual guided HPV testing quality for years.
• Rapid advancements necessitate an updated resource for professionals in HPV testing.

Purpose of the Study:

• To introduce the updated HPV Laboratory e-Manual.
• To provide a comprehensive, interactive resource for quality-assured HPV testing.
• To support research and HPV-based cervical cancer control.

Main Methods:

• Development by international experts from 11 countries.
• Chapters authored by two National HPV Reference Laboratories (NRLs) and reviewed by one.
• Content covers quality assurance, HPV taxonomy, specimen handling, nucleic acid extraction, detection, typing, serology, data management, and standards.

Main Results:

• The e-Manual provides up-to-date protocols and best practices for accurate and reliable HPV testing.
• Interactive features allow for real-time updates, creating a dynamic global resource.
• The e-Manual is freely accessible online.

Conclusions:

• The HPV Laboratory e-Manual promotes global harmonization of HPV testing methodologies.
• It serves as a vital tool for both research and cervical cancer control initiatives.
• This resource supports the WHO's goal of cervical cancer elimination.