Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Millimeter-Scale Single-Crystal α-MoO<sub>3</sub> Nanosheets Grown by Alkali Salt-Assisted Chemical Vapor Deposition.

ACS nano·2026

Same author

Controlled Growth of Rare-Earth-Doped TiO<sub>2</sub> Thin Films on III-V Semiconductors for Hybrid Quantum Photonic Interfaces.

ACS applied optical materials·2026

Same author

Engineering Nanohole-Etched Quantum Dots for Telecom-Band Single-Photon Generation.

ACS nano·2026

Same author

Nanoscale Infrared Spectroscopic Characterization of Extended Defects in 4H-Silicon Carbide.

Nano letters·2024

Same author

Elucidating the Mechanism of Large Phosphate Molecule Intercalation Through Graphene-Substrate Heterointerfaces.

ACS applied materials & interfaces·2023

Same author

Unraveling the Supramolecular Structure and Nanoscale Dislocations of Bacterial Cellulose Ribbons Using Correlative Super-Resolution Light and Electron Microscopy.

Biomacromolecules·2022

Search research articles

Related Experiment Video

Updated: Sep 16, 2025

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

High-energy resolution monochromated STEM-EELS mapping across large areas.

Caleb Whittier¹, Nabil D Bassim²

¹Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario L8S 4L7, Canada.

Ultramicroscopy

|July 12, 2025

Summary

Scanning transmission electron microscopy with electron energy loss spectroscopy (STEM-EELS) can now map large material areas with high energy resolution. This new method overcomes previous limitations for detailed chemical and optical property analysis.

Keywords:

Electron energy loss spectroscopy Low-loss EELS Monochromated EELS Phonons Plasmons Spectrum imaging

More Related Videos

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Published on: March 6, 2014

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

Related Experiment Videos

Last Updated: Sep 16, 2025

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Published on: March 6, 2014

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

Area of Science:

Materials Science
Analytical Chemistry
Spectroscopy

Background:

Scanning transmission electron microscopy (STEM) coupled with electron energy loss spectroscopy (EELS) offers high spatial resolution for material analysis.
Atomic-level material property investigation using EELS is advancing, but challenges remain for large-scale, high-energy resolution mapping.
Off-axis distortions in STEM-EELS limit mapping to small areas or compromise energy resolution over larger regions.

Purpose of the Study:

To develop a methodology for high-energy resolution STEM-EELS spectrum mapping over large areas (tens to hundreds of microns).
To overcome limitations imposed by off-axis distortions and aberrations in conventional STEM-EELS.
To enable detailed chemical and optical property analysis across extended material structures.

Main Methods:

Modification of EELS collection conditions, including careful alignment of scan/descan coils.
Implementation of elongated camera lengths to effectively magnify the object over the EELS entrance aperture.
Mitigation of higher-order aberrations and reduction of spectral shifts on the spectrometer.

Main Results:

Successful low-loss STEM-EELS spectrum mapping over tens to hundreds of microns.
Maintenance of high energy resolution across the mapped large areas.
Effective reduction of aberrations and spectral shifts caused by off-axis distortions.

Conclusions:

The proposed methodology significantly enhances the capability of STEM-EELS for large-scale material characterization.
This advancement allows for detailed analysis of chemical and optical properties over extended regions without sacrificing energy resolution.
The technique provides a powerful new tool for materials science research, enabling broader insights into material behavior.