Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

45.8K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
45.8K
Teratogenicity01:07

Teratogenicity

2.8K
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
2.8K
Nucleosome Remodeling02:54

Nucleosome Remodeling

9.5K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.5K
Gastrulation01:56

Gastrulation

58.6K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
58.6K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

5.5K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
5.5K
Fertilization01:38

Fertilization

72.4K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
72.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proband Nanopore Long-Read Genome Sequencing Facilitates Preimplantation Genetic Testing for Facioscapulohumeral Muscular Dystrophy.

Neurology. Genetics·2026
Same author

Serum Progesterone Level on the Day of Embryo Transfer Is Not a Reliable Predictor for Frozen-Thawed Embryo Transfer Outcomes With Euploid Blastocyst Transfer: A Retrospective Cohort Study.

BJOG : an international journal of obstetrics and gynaecology·2024
Same author

Clinical application of next generation sequencing-based haplotype linkage analysis in the preimplantation genetic testing for germline mosaicisms.

Orphanet journal of rare diseases·2023
Same author

Simultaneous detection of genomic imbalance in patients receiving preimplantation genetic testing for monogenic diseases (PGT-M).

Frontiers in genetics·2022
Same author

Evaluating the application value of NGS-based PGT-A by screening cryopreserved MDA products of embryos from PGT-M cycles with known transfer outcomes.

Journal of assisted reproduction and genetics·2022
Same author

Comparison of chromosomal status in reserved multiple displacement amplification products of embryos that resulted in miscarriages or live births: a blinded, nonselection case-control study.

BMC medical genomics·2022

Related Experiment Video

Updated: Sep 15, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.7K

Structural rearrangements affect blastocyst development.

Yizi Wang1,2,3, Yuanlin Ma1,2,3, Yanling Tan1,2,3

  • 1Reproductive Medicine Center, The First Affiliated Hospital, Sun Yat-Sen University, Yuexiu District, Zhongshan 2nd Road No. 1, Guangzhou, 510080, Guangdong, China.

Archives of Gynecology and Obstetrics
|July 12, 2025
PubMed
Summary

Chromosomal translocations, termed structural rearrangements (SR), lead to lower blastocyst formation and fewer usable embryos for transfer compared to monogenic disorders in preimplantation genetic testing (PGT) cycles.

Keywords:
Blastocyst developmentBlastocyst formationPreimplantation genetic testingStructural rearrangements

More Related Videos

Human Blastocyst Biopsy and Vitrification
10:59

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

22.8K
Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
08:28

Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps

Published on: February 28, 2019

8.8K

Related Experiment Videos

Last Updated: Sep 15, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.7K
Human Blastocyst Biopsy and Vitrification
10:59

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

22.8K
Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
08:28

Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps

Published on: February 28, 2019

8.8K

Area of Science:

  • Reproductive Medicine
  • Genetics
  • Embryology

Background:

  • Chromosomal translocations are debated for their impact on embryo development.
  • Preimplantation genetic testing (PGT) aids in selecting viable embryos.

Purpose of the Study:

  • To compare blastocyst formation in PGT cycles involving structural rearrangements (SR) versus monogenic disorders (PGT-M).
  • To assess the impact of SR on embryo development and transferability.

Main Methods:

  • Retrospective analysis of 791 PGT-SR cycles and 757 PGT-M cycles (Jan 2021 - May 2023).
  • Comparison of blastocyst formation rates and quality between the two groups.

Main Results:

  • PGT-SR cycles showed significantly lower blastocyst formation rates (≥3BB) compared to PGT-M.
  • Fewer day 5 blastocysts and a 12.7% reduction in eligible blastocysts were observed in the PGT-SR group.
  • The PGT-SR group had a substantially lower rate of transferrable balanced/normal blastocysts (32.3% vs. 59.9%).

Conclusions:

  • Patients with structural rearrangements experience diminished blastocyst development.
  • Significantly fewer usable blastocysts are available for transfer in PGT-SR cycles compared to PGT-M.
  • This highlights potential challenges in achieving successful implantation for individuals with SR.