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Updated: Sep 15, 2025
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
Published on: March 25, 2020
Protocol for studying the immune microenvironment of human hepatocellular carcinoma by Cell DIVE multiplex
Margaux Delaporte1, Maelle Guillout2, Pascale Bellaud2
1University Rennes, INSERM, EHESP, Irset - UMR_S 1085, 9 2 Avenue du Professeur Léon Bernard, 35000 Rennes, France.
Abstract:
Understanding tumor heterogeneity by examining how cells are organized in tissues is essential to better decipher the biological processes involved in cancer and to improve therapeutic management. Here, we present a protocol for a multiplex immunofluorescence (mIF) technique that enables the detection of an array of protein markers and allows immunophenotyping on a single section of human hepatocellular carcinoma. We describe the procedures for antibody labeling, mIF image acquisition using Cell DIVE, and preliminary analysis using QuPath software.
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