Related Experiment Video

Updated: Sep 15, 2025

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.1K

Combined Inhibition of XPO1 and DNA Methylation Exerts Synergistic Effects in DLBCL

Qi Li1, Xiaofeng Xue1, Si Chen2

  • 1Department of Hematology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

Molecular Carcinogenesis
|July 13, 2025
PubMed

Insights

Selinexor shows limited efficacy alone in diffuse large B-cell lymphoma (DLBCL). Combining selinexor with decitabine (DAC) demonstrates a synergistic antitumor effect, offering a promising new DLBCL treatment strategy.

Area of Science:

  • Oncology
  • Hematology
  • Molecular Biology

Background:

  • Diffuse large B-cell lymphoma (DLBCL) is an aggressive non-Hodgkin lymphoma with poor response to chemotherapy.
  • Selinexor, an XPO1 inhibitor, has shown antitumor activity but limited efficacy as monotherapy in DLBCL.
  • Elevated XPO1 expression in DLBCL correlates with poor patient outcomes.

Purpose of the Study:

  • To investigate the global impact of selinexor on DLBCL.
  • To explore combination strategies to enhance selinexor efficacy in DLBCL treatment.
  • To evaluate the synergistic effect of selinexor with decitabine (DAC) and lenalidomide (LEN).

Main Methods:

  • Comprehensive proteomic and transcriptomic analysis of DLBCL.
  • Evaluation of selinexor monotherapy and combination therapies (selinexor + DAC, selinexor + LEN).
  • Analysis of decitabine's mechanism in reversing selinexor-induced alterations.

Main Results:

  • Selinexor significantly impacts various biological processes in DLBCL.
  • Selinexor exhibits a synergistic effect with decitabine (DAC) against DLBCL.
  • DAC enhances selinexor's antitumor effect by reversing DNMT1 expression and DNA methylation changes.

Conclusions:

  • Selinexor combination therapy, particularly with DAC, is a promising strategy for DLBCL.
  • The combination overcomes limitations of selinexor monotherapy.
  • This approach holds significant potential for clinical application in DLBCL treatment.
Keywords:
DNA methylationXPO1combination therapydecitabinediffuse large B‐cell lymphoma

More Related Videos

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

646
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.7K

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
4.8K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.6K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.1K
Dosage Compensation02:50

Dosage Compensation

In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with  distinct numbers of X chromosomes will...
6.3K