Research on a single molecule signal amplification strategy for high risk HPV mRNA based on fluorescent microarray...

This study introduces a high-sensitivity single-molecule signal amplification approach for detecting high-risk HPV mRNA, utilizing a fluorescent microarray platform (FMP) based on modified entropy-driven chain substitution (EDC). Tailored for the high-throughput detection of 13 high-risk HPV types, the FMP enables concurrent identification of both single and multiple infections, including critical targets HPV16 and HPV18. Clinical validation revealed that the platform achieved over 90 % sensitivity and specificity, with detection sensitivity for HPV16 and HPV18 E6/E7 mRNA surpassing 95 % and specificity nearing 100 %. In samples with multiple HPV infections, the FMP accurately distinguished coexisting types without cross-reactivity, demonstrating its robustness for complex infection profiles. This fluorescence-based platform presents a scalable, precise solution for large-scale HPV screening and diagnostics, especially in resource-constrained environments.