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Updated: Sep 15, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
Prior human endemic coronavirus exposure does not affect humoral responses to SARS-CoV-2 protein vaccines
Guy de Bruyn1, Haritha Adhikarla2, Caroline K Brackett3
1Sanofi Vaccines Research & Development, Swiftwater, PA, USA. guy.debruyn@sanofi.com.
Abstract:
We included measurement of pre-existing immunity to human endemic coronaviruses (HCoV) in a Phase I/II study of SARS-CoV-2 spike protein vaccine candidates. A Binding Antibody Multiplex Assay measured HCoV-specific IgG to the receptor binding domain or full-length spike of human coronaviruses HKU1, 229E, NL63, and OC43. We found no evidence for the impact of HCoV antibodies on neutralizing and binding antibody responses to the candidate SARS-CoV-2 vaccines.
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