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Seasonal and Geographical Variability, Quantitative Analysis by RP-HPLC-PDA, and Anti-Obesogenic Potential of
Mridula Singh1, Akash Dey1, Dixita Rameshbhai Dhoriya1
1Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sahibzada Ajit Singh Nagar, Punjab, India.
Introduction:
Murraya koenigii contains bioactive compounds with antidiabetic and hypolipidemic activities. Considering widespread availability and traditional use of M. koenigii throughout South Asia.
Objectives:
The present study was designed to perform qualitative and quantitative analysis of phytochemicals present in curry leaves.
Material And Methods:
Quantification method was validated according to International Council of Harmonization (ICH) guidelines and was used to analyze seasonal and geographical variability of phytoconstituents in leaf samples collected from different geographical locations across India.
Results:
The isolated four carbazole alkaloids, namely, mahanimbine, girinimbine, koenimbine, and koenigicine, from methanolic extract of curry leaves, were quantitatively determined in the leaves of M. koenigii using reverse phase-high performance liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA).
Conclusions:
Our study revealed that mahanimbine, koenimbine, and koenigicine content was maximum in sample collected in months of April, June, and October 2020, respectively. The samples procured from Northern Indian regions showed maximum content of carbazole alkaloids. The in vitro biological assays revealed anti-obesogenic potential of carbazole alkaloids in 3T3-L1 adipocytes.
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