Related Experiment Video
Updated: Sep 15, 2025
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
Published on: June 19, 2015
Exploratory Study of CD10low Polymorphonuclear Leukocytes Preceding and Correlating With Postsurgical Inflammation
Timo Michael Westermann1, Joe Sendas1, Alexander Sebastian Koller1
1Institute of Clinical and Experimental Trauma Immunology, University Hospital Ulm, Ulm, Germany.
Abstract:
The lack of diagnostic and monitoring tools for postsurgical immunological complications such as systemic inflammation can contribute to a poor outcome despite modern intensive care. The need for reliable immune monitoring has been emphasised. Polymorphonuclear leukocytes (PMNs) play an important role in postsurgical inflammation. A subgroup of PMNs is particularly interesting, because they are released shortly after (iatrogenic) trauma: immature PMNs, characterised by, for example, their low CD10 expression. Therefore, we investigated the role of CD10low PMNs in a non-interventional exploratory study by including patients undergoing scheduled, highly standardised cardiac surgery with extracorporeal circulation. We were able to demonstrate that the number of CD10low PMNs released shortly after the beginning of surgery correlated with different fluid phase markers of inflammation and organ damage postsurgically. Among these parameters were CRP, IL-6, NGAL, CK-MB, and troponin-T. Noteworthy, the amount of CD10low PMNs increased as early as 24 h before these well-established markers, suggesting superiority of CD10low PMNs as an early diagnostic marker. Comparing CD10low immature PMNs with CD10high mature PMNs revealed potential involved mechanisms, including lower CD11b expression and a significant decrease in the formation of platelet-neutrophil complexes (PNCs) by CD10low PMNs. In conclusion, we propose CD10low PMNs as a potential early cellular biomarker to assess the postsurgical inflammatory response. In comparison to clinically established markers like CRP or IL-6 and scoring systems such as the SOFA-Score, CD10low PMNs reflect a potential candidate for future immune monitoring to determine the risk of excessive inflammation and organ impairment more rapidly.
Insights
Immature CD10-low polymorphonuclear leukocytes (PMNs) show potential as early biomarkers for postsurgical inflammation. Their numbers rise before established markers, aiding in rapid risk assessment for organ damage.
Area of Science:
- Immunology
- Biomarker Discovery
- Surgical Complications
Background:
- Postsurgical immunological complications, like systemic inflammation, lack effective diagnostic tools, impacting patient outcomes.
- Reliable immune monitoring is crucial for managing post-surgery inflammatory responses.
- Polymorphonuclear leukocytes (PMNs), particularly immature CD10-low PMNs, are implicated in early postsurgical inflammation.
Purpose of the Study:
- To investigate the role of CD10-low PMNs as an early diagnostic marker for postsurgical inflammation.
- To explore the correlation between CD10-low PMN counts and established inflammatory markers and organ damage indicators.
- To compare the characteristics of CD10-low PMNs with mature CD10-high PMNs.
Main Methods:
- Non-interventional exploratory study involving patients undergoing cardiac surgery with extracorporeal circulation.
- Quantification of CD10-low PMNs released post-surgery.
- Measurement of fluid phase markers of inflammation (CRP, IL-6) and organ damage (NGAL, CK-MB, troponin-T).
- Comparative analysis of CD10-low and CD10-high PMN expression (CD11b) and platelet-neutrophil complex (PNC) formation.
Main Results:
- The number of CD10-low PMNs correlated with postsurgical inflammation and organ damage markers.
- CD10-low PMN levels increased significantly 24 hours earlier than established markers like CRP and IL-6.
- CD10-low PMNs exhibited lower CD11b expression and reduced formation of platelet-neutrophil complexes compared to CD10-high PMNs.
Conclusions:
- CD10-low PMNs represent a promising early cellular biomarker for assessing postsurgical inflammatory response.
- These immature PMNs may offer superior early detection of inflammation compared to current markers.
- CD10-low PMNs could facilitate rapid immune monitoring for excessive inflammation and organ impairment risk.
More Related Videos
09:00Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
Published on: June 24, 2020
10:29Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
Published on: August 14, 2019