Advanced Injectable Human-Derived Microgels for Improved Cell Delivery and Tissue Regeneration

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Tissue Engineering

Background:

• Cell delivery therapies face challenges in cell viability and retention post-injection.
• Existing hydrogels often use animal-derived materials (immunogenicity) or synthetic polymers (poor cell adhesion).

Purpose of the Study:

• To develop injectable, human protein-derived microgels for enhanced cell delivery.
• To create a cell carrier that supports cell viability and promotes controlled cell migration.

Main Methods:

• Fabrication of methacryloyl platelet lysate microgels using a solvent-free method on superhydrophobic surfaces via light irradiation.
• Incorporation of collagenase to facilitate enzyme-mediated cell migration.
• Assessment of injectability, cell viability, morphology, and defect-filling capacity in porcine tissue.
• Modification with magnetic nanoparticles for spatial control.

Main Results:

• Human protein-derived microgels successfully sustained cell viability and promoted mesenchymal stem cell migration.
• Enzyme-mediated degradation allowed controlled outward cell movement without compromising viability.
• Microgels demonstrated injectability, preserved morphology, and effectively filled irregular tissue defects.
• Magnetic nanoparticle incorporation enabled external magnetic field control.

Conclusions:

• Injectable human protein microgels are a viable platform for cell delivery, overcoming limitations of current therapies.
• The developed microgels support cell viability, enhance migration, and offer tunable properties for personalized cell therapies.
• The technology shows potential for improved therapeutic outcomes in regenerative medicine and tissue repair.