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Related Concept Videos

Development of the Heart01:27

Development of the Heart

The development of the human heart, a crucial organ, commences from the mesoderm on the 18th or 19th day after fertilization. This process initiates in the cardiogenic area, a group of mesodermal cells at the embryo's head end, which evolves into elongated strands known as cardiogenic cords. These cords undergo a transformation to form hollow-centered endocardial tubes.
As the embryo undergoes lateral folding, these paired tubes approach each other, merging into a single primitive heart tube by...

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Related Experiment Video

Updated: Jun 20, 2026

In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells
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Heart spheroids from primary cells for studying cardiac development.

Cindy Chang, Juan Xu, Shirley Chang

    Biorxiv : the Preprint Server for Biology
    |July 14, 2025
    PubMed
    Summary

    Researchers developed novel heart spheroids using primary murine cells, preserving in vivo characteristics for disease modeling. This primary cell-derived approach offers a promising alternative to stem cell methods for translational research.

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    Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
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    Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

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    Area of Science:

    • Cardiovascular Biology
    • Regenerative Medicine
    • Organoid Technology

    Background:

    • Organoids are valuable for disease modeling and drug screening.
    • Primary cell-derived organoids exist for liver, intestine, and colon, but not for the heart.
    • Stem cell-derived organoids are an alternative but may not fully preserve native cellular features.

    Purpose of the Study:

    • To develop primary cell-derived heart organoids (spheroids) from murine atrial and ventricular cells.
    • To characterize their in vivo-like properties and responses to stimuli.
    • To explore their potential for translational research.

    Main Methods:

    • Generation of heart organoid-like spheroids from primary murine atrial and ventricular cells.
    • Evaluation of cell lineage preservation and response to growth factors.
    • Fusion of different spheroid types to study cell migration and interaction.
    • Fibroblast ablation assay in transgenic mouse-derived heart spheroids.

    Main Results:

    • Murine heart spheroids largely preserved in vivo cell lineages.
    • Spheroids responded to growth factor treatments.
    • Region- and organ-specific cells clustered together in mixed spheroids.
    • Co-culture with liver and intestine cells promoted cardiomyocyte maturation.
    • Fibroblast ablation affected other cell lineages within the spheroids.

    Conclusions:

    • Successfully generated primary cell-derived heart spheroids with in vivo-like characteristics.
    • This method preserves native cellular features, making it promising for translational research.
    • Primary cell-derived heart organoids offer advantages over stem cell-derived systems for specific applications.