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Updated: Sep 15, 2025
12:00
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
Published on: April 8, 2009
10.4K
Comprehensive Genomic, Mutation, Phylogenetic, and Statistical Analysis of the Monkeypox Virus Across Multiple
Imran Zafar1, Faheem Kanwal2, Muhammad Azmat2
1Department of Biochemistry and Biotechnology, Faculty of Science, The University of Faisalabad (TUF), Faisalabad, Pakistan.
Indian Journal of Microbiology
|July 14, 2025
Summary
This study analyzes Monkeypox virus (MPXV) genomics and epidemiology, identifying key mutations and vulnerable proteins. Findings inform public health strategies and potential therapeutics for MPXV prevention and control.
Area of Science:
- Virology
- Genomics
- Epidemiology
Background:
- Monkeypox virus (MPXV) poses a significant public health threat, necessitating comprehensive analysis.
- Understanding MPXV's genetic diversity, transmission, and clinical features is crucial for effective control.
Purpose of the Study:
- To conduct a comprehensive analysis of Monkeypox virus (MPXV), exploring its epidemiology, genomics, and potential vulnerabilities.
- To identify novel insights into MPXV's mutation landscape, host interactions, and therapeutic targets.
Main Methods:
- Genome data analysis (NCBI, 2019-2023) including sequence alignment, protein identification, and phylogenomic analysis.
- Integration of clinical data, geospatial mapping, statistical analysis, and artificial intelligence/machine learning (AI/ML).
- In-silico studies for identifying potential drug targets and exploring plant-based treatments.
Main Results:
- Identification of unique MPXV lineages and a specific mutation linked to human transmission.
- Pinpointed 24 MPXV proteins as highly mutation-prone, with domain analysis revealing conserved Ortho-poxvirus genes.
- AI/ML models provided predictive insights into MPXV progression, and potential drug targets were identified.
Conclusions:
- The study provides a multi-faceted understanding of MPXV, integrating genomic and epidemiological data.
- Findings support the development of real-time preventive measures and optimized public health responses.
- Identified mutation-prone proteins and potential therapeutic targets offer avenues for future MPXV interventions.
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