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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
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A Method for Temporally Resolved Continuous Inline Measurement of Multiple Solute Concentrations With Microfluidic

Andrea Lorenzo Henri Sergio Detry1,2, Vinny Chandran Suja3,4, Nathaniel Merriman Sims2

  • 1Department of Chemical, Materials, Industrial Production EngineeringUniversity of Naples Federico II 80125 Naples Italy.

IEEE Open Journal of Engineering in Medicine and Biology
|July 14, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a compact microfluidic spectroscopy system for real-time, simultaneous measurement of multiple solute concentrations in dynamic fluid flow. The technology offers high precision and portability for advanced fluid analysis.

Keywords:
Inline measurementsmicrofluidicsmulti-fluid analysisreal-time monitoringspectroscopy

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Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Spectroscopy

Background:

  • Traditional fluid analysis methods often lack real-time, multi-solute detection capabilities.
  • High temporal resolution is crucial for understanding dynamic processes in fluid pathways.

Purpose of the Study:

  • To develop a compact, real-time microfluidic spectroscopy system for simultaneous multi-solute concentration measurement.
  • To achieve high temporal resolution in monitoring solutes within a single fluid pathway.

Main Methods:

  • Integration of a Z-flow cell, dual-wavelength LEDs, and a compact spectrophotometer.
  • Utilized clinical infusion pumps and a catheter for fluid delivery and mixing of marker dyes.
  • Employed high-frequency sampling and MATLAB for automated data acquisition and control.

Main Results:

  • The system accurately measured solute concentrations with high precision and reproducibility.
  • Demonstrated low standard error of the mean for Erioglaucine and Tartrazine.
  • Achieved high accuracy with minimal deviation from expected steady-state concentrations.

Conclusions:

  • The developed system enables continuous, real-time monitoring of multiple solutes in dynamic flow.
  • Offers a portable solution with high sensitivity to temporal concentration changes.
  • Represents an advancement over traditional static fluid measurement techniques.