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Optimized Electrochemical Sensor for Ochratoxin A Quantification in Coffee and Wheat Matrices.

Jane S de Medeiros1, Jairo P Oliveira2, Helder Knidel3

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A new electrochemical method offers sensitive ochratoxin A (OTA) detection in food. This cost-effective approach using modified screen-printed electrodes (SPE) provides reliable results for food safety monitoring.

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Electrochemistry

Background:

  • Ochratoxin A (OTA) is a prevalent mycotoxin in food, posing significant health risks.
  • Accurate and sensitive detection of OTA in complex food matrices is crucial for public health and regulatory compliance.
  • Existing methods for OTA detection can be costly, time-consuming, and suffer from matrix interferences.

Purpose of the Study:

  • To develop a cost-effective, sensitive, and selective electrochemical method for OTA detection.
  • To optimize the method for challenging food matrices like wheat and coffee.
  • To validate the method's performance against regulatory standards.

Main Methods:

  • Utilized commercial carbon black-graphite screen-printed electrodes (SPE CB-G) modified with cetyltrimethylammonium bromide (CTAB).
  • Employed adsorptive stripping differential pulse voltammetry (AdSDPV) with parameters optimized via central composite design (CCD).
  • Evaluated method performance in buffer solutions and real food samples (wheat, coffee).

Main Results:

  • Achieved a low limit of detection (LOD) of 1.39 ng mL⁻¹ in buffer and 2.70 ng mL⁻¹ in coffee.
  • Demonstrated high recovery rates (94.64–109.86%) in coffee, indicating accuracy.
  • CTAB modification effectively minimized matrix effects, ensuring reliable and reproducible results.

Conclusions:

  • The optimized SPE-based electrochemical sensor provides a practical and efficient alternative for routine OTA analysis.
  • This method offers a cost-effective solution for sensitive and selective OTA detection in food safety monitoring.
  • The developed sensor meets regulatory requirements and shows promise for widespread application in food quality control.