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Related Concept Videos

Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

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Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
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Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain
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Heat-killed Bacillus subtilis concerning broilers' performance, cecal architecture and microbiota.

Yan Cui1,2, Weishuang Meng3, Feng He1,2

  • 1College of Husbandry and Veterinary Medicine, Jinzhou Medical University, Jinzhou, China.

Frontiers in Microbiology
|July 14, 2025
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Summary

Dietary supplementation with heat-killed Bacillus subtilis (HKB) improved broiler growth performance and gut health. HKB enhanced body weight gain, feed efficiency, and beneficial gut morphology by modulating cecal microbial composition.

Keywords:
ITSadditivehindgutintestinal fungus compositionintestinal healthpostbiotic

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Area of Science:

  • Animal Science
  • Microbiology
  • Gut Health

Background:

  • Bacillus subtilis is recognized as a probiotic feed additive for broilers.
  • Limited research exists on the efficacy of Bacillus subtilis-derived postbiotics.

Purpose of the Study:

  • To investigate the effects of heat-killed Bacillus subtilis (HKB) on broiler growth, cecal morphology, and microbial composition.
  • To determine dose-dependent responses to HKB supplementation.

Main Methods:

  • 480 Arbor acre broiler chicks were divided into four groups (0, 0.015, 0.030, 0.045% HKB) for 42 days.
  • Evaluated growth performance, cecal morphology (villus height, crypt depth), and cecal microbial composition (16S rRNA for bacteria, ITS for fungi).

Main Results:

  • HKB supplementation dose-dependently improved body weight gain and gain-to-feed ratio.
  • Enhanced cecal morphology with increased villus height and villus-to-crypt ratio, and reduced crypt depth.
  • Reduced cecal bacteria diversity (Chao1 index) and modulated bacterial and fungal composition.

Conclusions:

  • Dietary HKB effectively improves broiler growth performance.
  • HKB enhances cecal morphology and modulates gut microbiota composition.
  • Heat-killed Bacillus subtilis is a viable postbiotic for improving broiler production.