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Updated: Sep 15, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Association between Gut Microbiota Composition and Copy Number Variations in Human Genes FAM66D and TAS2R43
Jing Wang1, Haoyu Guo1, Weiwei Qi1
1Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, P.R. China.
Abstract:
The impact of the gut microbiota on human health has attracted increasing attention. However, the factors affecting the gut microbiota need more in-depth research. In this work, we analyzed the impact of the gut microbiota at the genetic levels, mainly gene copy number variations (CNVs). We used Permutational Multivariate Analysis of Variance (PERMANOVA, Adonis) analysis to assess the association between CNVs and the gut microbiota. The results showed that copy numbers of 50 genes varied among the cohort. Among them, the CNVs of 7 genes, including TBC1D3L, OR4C6, NPIPB15, PDPR, USP17L7, FAM66D and TAS2R43, were related to the gut microbiota. In addition, among these genes, we systematically analyzed the relationship between CNVs of FAM66D and TAS2R43 with the gut microbiota. We hypothesize that there exists a certain association between genetic information in the human genome (such as CNVs) and human behavior as well as gut microbiota.
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