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The function of TaWOX14 in wheat genetic transformation
Dan Wang1, Yajie Guo1, Mengtian Liu1
1College of Agronomy and the State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, Henan, China.
Plant Cell Reports
|July 14, 2025
Summary
Overexpression of wheat TaWOX14 enhances genetic transformation and genome editing. TaPLD is identified as a potential gene for haploid induction, accelerating wheat breeding advancements.
Area of Science:
- Plant Science
- Genetics
- Agricultural Biotechnology
Background:
- Genetic transformation and genome editing are crucial for crop improvement but often limited by explant regeneration efficiency.
- The WUSCHEL-related homeobox (WOX) gene family, particularly the WUS clade, is known to be vital for somatic embryogenesis in plants.
- Improving regeneration efficiency is key to accelerating crop breeding programs.
Purpose of the Study:
- To investigate the role of WUS clade genes in wheat transformation.
- To enhance genetic transformation and genome editing efficiencies in wheat using TaWOX14.
- To explore TaPLD as a candidate gene for haploid induction in wheat.
Main Methods:
- Systematic analysis of WUS clade genes in wheat transformation.
- Overexpression of TaWOX14 in wheat immature embryos across multiple genotypes.
- Combination of CRISPR-Cas9 system with TaWOX14 for genome editing.
- Editing of wheat homologs of Zea mays PHOSPHOLIPASE D3 (ZmPLD3) to identify TaPLD's role in haploid induction.
Main Results:
- Overexpression of TaWOX14 significantly improved genetic transformation efficiencies in wheat genotypes Fielder, Kenong199, Zhengmai7698, and Yangmai13.
- Enhanced genome editing efficiency was observed in wheat variety Fielder when using the CRISPR-Cas9 system combined with TaWOX14.
- A TaPLD-edited plant with an intermediate ploidy level between haploid and hexaploid was identified, suggesting its potential for haploid induction.
Conclusions:
- TaWOX14 overexpression is a viable strategy to significantly improve wheat genetic transformation and genome editing efficiencies.
- TaPLD emerges as a promising candidate gene for facilitating haploid induction in wheat.
- These findings collectively contribute to accelerating wheat breeding through enhanced transformation and novel haploid induction methods.
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