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A Multimodal Adaptive Optical Microscope For In Vivo Imaging from Molecules to Organisms.
Tian-Ming Fu1, Gaoxiang Liu2, Daniel E Milkie1
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
Biorxiv : the Preprint Server for Biology
|July 15, 2025
Summary
Researchers developed MOSAIC, a versatile microscope integrating multiple advanced imaging techniques. This reconfigurable platform enables non-invasive, multi-scale biological imaging from subcellular dynamics to whole organisms.
Area of Science:
- Biophysics
- Microscopy
- Cell Biology
Background:
- Observing biological systems requires multi-scale imaging across space and time.
- Current microscopes face limitations in versatility and optical aberrations in complex samples.
Purpose of the Study:
- To present MOSAIC, a reconfigurable microscope integrating multiple advanced imaging techniques.
- To enable non-invasive, multi-scale imaging of biological processes.
Main Methods:
- Integration of light-sheet, label-free, super-resolution, and multi-photon microscopy.
- Incorporation of adaptive optics for aberration correction.
- Development of a reconfigurable platform for diverse imaging needs.
Main Results:
- MOSAIC enables non-invasive imaging of subcellular dynamics in cells and organisms.
- Facilitates nanoscale mapping of molecular architectures in expanded tissues.
- Supports structural and functional neural imaging in live mice.
Conclusions:
- MOSAIC provides an integrated platform for comprehensive biological investigation.
- The system facilitates correlative studies across diverse biological scales within the same specimen.
