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Filopodia numbers impact chemotactic migration speed.

Annika L Schroder1,2, Livia D Songster1,3, Yahor Savich4,5

  • 1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455.

Biorxiv : the Preprint Server for Biology
|July 15, 2025
PubMed
Summary
This summary is machine-generated.

Filopodia, cell protrusions, are not essential for amoebas to detect chemical signals but influence migration speed. Changes in filopodia number affect how fast amoeboid cells move.

Keywords:
actinamoeboid migrationchemotaxisdirected migrationfilopodiamyosin

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Area of Science:

  • Cell Biology
  • Biophysics
  • Developmental Biology

Background:

  • Migrating cells navigate using external cues.
  • Filopodia are cell protrusions involved in environmental interaction.
  • The function of filopodia in chemotaxis of fast-moving amoeboid cells is understudied.

Purpose of the Study:

  • Investigate the role of filopodia in chemotactic guidance in the social amoeba Dictyostelium.
  • Determine how filopodia formation is regulated by chemoattractants.
  • Assess the impact of filopodia on amoeboid cell migration speed and directionality.

Main Methods:

  • Stimulation of Dictyostelium amoebae with chemoattractant cAMP.
  • Observation of filopodia formation and dynamics.
  • Analysis of cell migration in wildtype and mutant Dictyostelium lacking the filopodial myosin DdMyo7.
  • Quantification of filopodia number and cell migration speed.

Main Results:

  • Chemoattractant stimulation transiently increases filopodia formation, involving DdMyo7.
  • Filopodia formation is biased towards the chemoattractant source.
  • Cells lacking filopodia (myo7 null) show normal directional migration.
  • Altered filopodia numbers (lacking or increased) lead to slower migration speeds.

Conclusions:

  • Filopodia are dispensable for detecting chemical gradients in Dictyostelium chemotaxis.
  • Filopodia number, rather than their presence, influences amoeboid cell migration speed.
  • Changes in filopodia number may affect migration speed by altering cell-substrate adhesion.