Related Experiment Video
Updated: Sep 15, 2025
05:18
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
9.8K
Method Comparison and Investigation of Interference Variables of a Hand-Held Hemoglobinometer(HemoCue Hb 201+) in
Yuqing Sun1, Xiu Ting Yiew1, Janet Beeler-Marfisi2
1Departments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Veterinary Clinical Pathology
|July 15, 2025
Summary
The HemoCue Hb 201+ (HC-201) provides reliable point-of-care hemoglobin measurements in cats, with minimal bias and excellent agreement compared to laboratory methods. This device aids in timely clinical decisions for anemic feline patients.
Area of Science:
- Veterinary Hematology
- Point-of-Care Diagnostics
- Clinical Pathology
Background:
- Monitoring anemic cats requires frequent blood sampling, risking further anemia.
- Laboratory hemoglobin results can cause delays in critical clinical decisions.
- The HemoCue Hb 201+ (HC-201) offers rapid, low-volume blood analysis for hemoglobin.
Purpose of the Study:
- Evaluate the diagnostic utility of the HC-201 for feline hemoglobin.
- Assess the impact of common blood interferents on HC-201 performance.
- Determine the reliability of HC-201 for point-of-care use in cats.
Main Methods:
- Compared HC-201 hemoglobin measurements to a reference analyzer (ADVIA 2120) using 154 samples from 93 cats.
- Assessed agreement and bias using Lin's concordance correlation coefficient, Bland-Altman, and Passing-Bablok regression.
- Evaluated HC-201 performance in the presence of anemia, leukocytosis, azotemia, lipemia, and different venipuncture sites.
Main Results:
- HC-201 showed excellent agreement (ρc=0.989) and precision (ICC=0.997) with the reference method.
- A small constant bias was detected, but no significant proportional bias was observed.
- Anemia, leukocytosis, azotemia, lipemia, and venipuncture site did not affect HC-201 results.
Conclusions:
- The HC-201 is a reliable point-of-care device for measuring hemoglobin concentration in cats.
- Its accuracy and precision support its use in clinical settings for rapid diagnostic assessment.
- This technology can improve efficiency in managing feline anemia and other conditions requiring hemoglobin monitoring.
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