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Automated assignment grading with large language models: insights from a bioinformatics course
Pavlin G Poličar1, Martin Špendl1, Tomaž Curk1
1Faculty of Computer and Information Science, University of Ljubljana, Večna pot 113, 1000 Ljubljana, Slovenia.
Bioinformatics (Oxford, England)
|July 15, 2025
Summary
Large language models (LLMs) can effectively grade student assignments, providing feedback comparable to human teaching assistants. This technology offers a scalable solution for personalized education, with open-source LLMs proving as capable as commercial options.
Area of Science:
- Educational Technology
- Artificial Intelligence in Education
- Bioinformatics Education
Background:
- Individualized feedback is crucial for student learning but challenging to provide at scale.
- Large language models (LLMs) offer a potential solution for efficient, personalized feedback.
- Evaluating LLM effectiveness in real educational settings is essential.
Purpose of the Study:
- To evaluate the practical effectiveness of LLM-based grading for written assignments.
- To compare the grading accuracy and feedback quality of LLMs against human teaching assistants (TAs).
- To assess the performance of both commercial and open-source LLMs.
Main Methods:
- A practical evaluation was conducted in an "Introduction to Bioinformatics" course with over 100 students.
- Students' text-based answers were graded using LLMs, with a subset receiving feedback from both LLMs and human TAs in a blind study.
- Six commercial and open-source LLMs were systematically evaluated and compared to human TA performance.
Main Results:
- LLMs, when prompted effectively, achieved grading accuracy and feedback quality comparable to human TAs.
- Open-source LLMs demonstrated performance on par with commercial LLMs.
- Student feedback quality ratings were similar for LLM-generated and human-generated feedback.
Conclusions:
- LLMs are a viable tool for grading written assignments, offering scalable and high-quality feedback.
- Open-source LLMs provide a cost-effective and privacy-preserving alternative for educational institutions.
- LLM-based grading can reduce instructor workload while enhancing student learning experiences.

