Analysis of a Near-Missing RhDV Type 1 Detection.

Area of Science:

• Hematology
• Immunology
• Genetics

Background:

• Blood transfusion safety is challenged by complex blood group antigens, particularly the Rh blood group system.
• Accurate identification and management of RhD blood groups are essential for clinicians and laboratory personnel.

Observation:

• A Chinese female patient with cervical squamous cell carcinoma presented with serological type A blood and varying RhD titers.
• RHD genetic testing was conducted to investigate the discrepancies in RhD typing.

Findings:

• RHD gene sequencing confirmed the patient as RhDV Type 1.
• Transfusion of type A RhD-negative red blood cells successfully increased hemoglobin levels without adverse reactions.

Implications:

• This case underscores the need for diverse RhD antibody reagents to accurately identify RhD subtypes.
• Precise RhD typing is critical for ensuring patient safety in blood transfusions, especially in complex cases.