Coupling Organic Ultralong Lifetime Phosphorescence Materials with Paper-Based Bioanalytical Architectures for...

Area of Science:

• Analytical Chemistry
• Materials Science
• Biotechnology

Background:

• Minimizing autofluorescence is critical for sensitive fluorescence-based bioanalytical assays.
• Conventional persistent luminescence phosphors often rely on rare earth elements and complex synthesis.
• There is a need for simpler, cost-effective materials for autofluorescence-free sensing.

Purpose of the Study:

• To introduce a novel sensor platform utilizing the ultralong phosphorescence of organic pyrene-doped benzophenone.
• To demonstrate the adaptability of this platform for various chemo- and biosensing applications.
• To provide a practical framework for integrating organic ultralong phosphorescent materials into bioanalytical devices.

Main Methods:

• Melt-casting pyrene-doped benzophenone onto cellulose paper to create a modular sensor platform.
• Developing optode membranes for "glow-in-the-dark" detection of potassium (K+) and sodium (Na+) ions.
• Adapting enzymatic platforms for hydrogen peroxide, glucose, and lactate sensing via peroxidase activity.
• Creating a paper-based enzyme-linked immunosorbent assay (ELISA) for immunorecognition.

Main Results:

• Demonstrated successful "glow-in-the-dark" detection of K+ and Na+ ions using optode membranes.
• Successfully adapted enzymatic assays for H2O2, glucose, and lactate detection by modulating phosphorescence emission.
• Developed a functional paper-based ELISA for immunorecognition applications.
• The organic ultralong phosphorescent material enabled autofluorescence-free quantification.

Conclusions:

• The pyrene-doped benzophenone platform offers a simple, adaptable, and low-cost approach for creating autofluorescence-free bioanalytical sensors.
• This organic ultralong phosphorescence material shows significant potential for integration into diverse receptor-based sensor designs.
• The developed sensor platform facilitates sensitive detection in various applications, from ion monitoring to complex immunoassays.